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aav9 syn flex jgcamp7f  (Addgene inc)


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    Addgene inc aav9 syn flex jgcamp7f
    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
    Aav9 Syn Flex Jgcamp7f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 160 article reviews
    aav9 syn flex jgcamp7f - by Bioz Stars, 2026-10
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    1) Product Images from "Basal ganglia output dynamically controls skilled forelimb kinematics in real time"

    Article Title: Basal ganglia output dynamically controls skilled forelimb kinematics in real time

    Journal: bioRxiv

    doi: 10.64898/2026.03.09.710687

    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber photometry strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
    Figure Legend Snippet: (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber photometry strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.

    Techniques Used: Sequencing, Blocking Assay, Injection, Activity Assay

    (A) Schematic of the miniscope recording strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to monitor calcium dynamics in GABAergic projection neurons at single-cell resolution during behavior. (B) Average population activity aligned to the onset of the Reach motif. The solid line represents the mean, and the shaded area represents SEM. (C) Heatmap of trial-averaged, Reach-aligned calcium activity for all recorded neurons. (D) Proportion of neurons exhibiting significantly increased (orange), decreased (purple), or unchanged (gray) activity during specific action motifs compared to baseline periods (Wilcoxon Signed-Rank Test). (E) Heatmap of the change in activity (ΔZscore; Behavior - Baseline) for all neurons across the six action motifs, sorted in descending order by modulation magnitude during the Retract motif. (F) Representative temporal traces comparing actual (orange) and predicted (light purple) forelimb spatial coordinates (X, Y, Z) and velocity. Predictions were generated using a linear regression model trained on concurrent SNr population activity. (G) Quantification of the linear regression model’s performance ($R^2$ score) in predicting moment-by-moment kinematics across sessions. Violin plots display the full data distribution density, with internal box plots representing the median (center marker) and the interquartile range. (H) Heatmap of normalized regression weights for each neuron across the four predicted kinematic features. Neurons (rows) are sorted in descending order by their assigned weight in the velocity model. n = 315 neurons from 600 trials, 10 sessions, 5 mice.
    Figure Legend Snippet: (A) Schematic of the miniscope recording strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to monitor calcium dynamics in GABAergic projection neurons at single-cell resolution during behavior. (B) Average population activity aligned to the onset of the Reach motif. The solid line represents the mean, and the shaded area represents SEM. (C) Heatmap of trial-averaged, Reach-aligned calcium activity for all recorded neurons. (D) Proportion of neurons exhibiting significantly increased (orange), decreased (purple), or unchanged (gray) activity during specific action motifs compared to baseline periods (Wilcoxon Signed-Rank Test). (E) Heatmap of the change in activity (ΔZscore; Behavior - Baseline) for all neurons across the six action motifs, sorted in descending order by modulation magnitude during the Retract motif. (F) Representative temporal traces comparing actual (orange) and predicted (light purple) forelimb spatial coordinates (X, Y, Z) and velocity. Predictions were generated using a linear regression model trained on concurrent SNr population activity. (G) Quantification of the linear regression model’s performance ($R^2$ score) in predicting moment-by-moment kinematics across sessions. Violin plots display the full data distribution density, with internal box plots representing the median (center marker) and the interquartile range. (H) Heatmap of normalized regression weights for each neuron across the four predicted kinematic features. Neurons (rows) are sorted in descending order by their assigned weight in the velocity model. n = 315 neurons from 600 trials, 10 sessions, 5 mice.

    Techniques Used: Injection, Single Cell, Activity Assay, Generated, Marker

    Related Articles

    Mouse Assay:

    Article Title: Nucleus Accumbens Local Circuit for Cue-Dependent Aversive Learning
    Article Snippet: .. Mice received bilateral stereotactic injections (0.3-0.5 μL) of either AAV9-hsyn-GRAB-ACh3.0 (Addgene; plasmid# 121922; packaged by Vigene Biosciences) , AAV9-syn-FLEX-jGCaMP7f (Addgene; 104492-AAV9), AAV5-EF1a-DIO-eNpHR3.0-eYFP (Addgene; 26966-AAV5), or AAV1-mU6-Tac1r-gRNA1::EF1a-NucEnv-EGFP::hU6-Tacr1-gRNA2 in the NAc core (created and packaged by NIDA GEVVC Core) (A/P: 1.6, Lat: 1.6, D/V: −4.4, 10° angle). ..

    Article Title: Nucleus accumbens local circuit for cue-dependent aversive learning
    Article Snippet: .. Mice received bilateral stereotactic injections (0.3–0.5 μL) of either AAV9-hsyn-GRAB-ACh3.0 (Addgene; plasmid# 121922; packaged by Vigene Biosciences), AAV9- syn -FLEX-jGCaMP7f (Addgene; 104492-AAV9), AAV5-EF1a-DIO-eNpHR3.0-eYFP (Addgene; 26966-AAV5), or AAV1-mU6-Tac1r-gRNA1EF1a-NucEnv-EGFPhU6-Tacr1-gRNA2 in the NAc core (created and packaged by NIDA GEVVC Core) (A/P: 1.6, Lat: 1.6, D/V: −4.4, 10° angle). ..

    Plasmid Preparation:

    Article Title: Nucleus Accumbens Local Circuit for Cue-Dependent Aversive Learning
    Article Snippet: .. Mice received bilateral stereotactic injections (0.3-0.5 μL) of either AAV9-hsyn-GRAB-ACh3.0 (Addgene; plasmid# 121922; packaged by Vigene Biosciences) , AAV9-syn-FLEX-jGCaMP7f (Addgene; 104492-AAV9), AAV5-EF1a-DIO-eNpHR3.0-eYFP (Addgene; 26966-AAV5), or AAV1-mU6-Tac1r-gRNA1::EF1a-NucEnv-EGFP::hU6-Tacr1-gRNA2 in the NAc core (created and packaged by NIDA GEVVC Core) (A/P: 1.6, Lat: 1.6, D/V: −4.4, 10° angle). ..

    Article Title: Nucleus accumbens local circuit for cue-dependent aversive learning
    Article Snippet: .. Mice received bilateral stereotactic injections (0.3–0.5 μL) of either AAV9-hsyn-GRAB-ACh3.0 (Addgene; plasmid# 121922; packaged by Vigene Biosciences), AAV9- syn -FLEX-jGCaMP7f (Addgene; 104492-AAV9), AAV5-EF1a-DIO-eNpHR3.0-eYFP (Addgene; 26966-AAV5), or AAV1-mU6-Tac1r-gRNA1EF1a-NucEnv-EGFPhU6-Tacr1-gRNA2 in the NAc core (created and packaged by NIDA GEVVC Core) (A/P: 1.6, Lat: 1.6, D/V: −4.4, 10° angle). ..

    Imaging:

    Article Title: Basal ganglia output dynamically controls skilled forelimb kinematics in real time
    Article Snippet: .. For miniscope calcium imaging, 100 nL of AAV9-Syn-FLEX-jGCaMP7f (Addgene #104488) was injected unilaterally into the left SNr. .. A GRIN lens (6 mm x 0.5 mm; Inscopix) was implanted above the left SNr (AP −3.7 mm, ML −1.5 mm, DV −4.4 mm) and secured with dental acrylic.

    Injection:

    Article Title: Basal ganglia output dynamically controls skilled forelimb kinematics in real time
    Article Snippet: .. For miniscope calcium imaging, 100 nL of AAV9-Syn-FLEX-jGCaMP7f (Addgene #104488) was injected unilaterally into the left SNr. .. A GRIN lens (6 mm x 0.5 mm; Inscopix) was implanted above the left SNr (AP −3.7 mm, ML −1.5 mm, DV −4.4 mm) and secured with dental acrylic.



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    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
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    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
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    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
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    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
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    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
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    Addgene inc aav9
    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
    Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    aav9 - by Bioz Stars, 2026-10
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    bacterial and virus strains aav9-syn-flex-jgcamp7f-wpre  (Addgene inc)
    90
    Addgene inc bacterial and virus strains aav9-syn-flex-jgcamp7f-wpre
    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber <t>photometry</t> <t>strategy.</t> <t>AAV9-Syn-FLEX-jGCaMP7f</t> was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.
    Bacterial And Virus Strains Aav9 Syn Flex Jgcamp7f Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav9+syn+flex+jgcamp7f/mcherry/pm40086431-264-2-13
    Average 90 stars, based on 1 article reviews
    bacterial and virus strains aav9-syn-flex-jgcamp7f-wpre - by Bioz Stars, 2026-10
    90/100 stars
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    Image Search Results


    (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber photometry strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.

    Journal: bioRxiv

    Article Title: Basal ganglia output dynamically controls skilled forelimb kinematics in real time

    doi: 10.64898/2026.03.09.710687

    Figure Lengend Snippet: (A) Schematic of the free-moving behavioral setup utilizing two perpendicular high-speed cameras for simultaneous recording and 3D reconstruction of forelimb reaching kinematics. (B) Decomposition of the reaching sequence into six distinct, stereotyped action motifs ( Lift , Reach , Open , Grasp , Retract , Drink ). Top and bottom rows show orthogonal views of the posture for each motif. Colors correspond to specific motifs throughout the figure (color legend inset). Trial-based task structure illustrated above. (C) Representative motif ethogram (top) and corresponding motif probability (bottom) from an example session (150 trials), aligned to the first Open motif. (D) Motif transition matrix from the representative session in (C). Each color block represents the transition probability from the motif on the y-axis to the motif on the x-axis. (E) Reconstructed 3D trajectories of the forelimb for all trials in the representative session in (C), color-coded by motif. (F) Schematic of the fiber photometry strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to record populational calcium dynamics in SNr GABAergic neurons during behavior. (G) Representative heatmaps of Z-scored SNr calcium signals from ipsilateral (left) and contralateral (right) hemispheres, aligned to water delivery and sorted by the onset of the first Open motif (pink line). Error bars represent mean ± SEM. (H) Average Z-scored SNr activity aligned to water delivery, stratified as early reaching trials and late reaching trials by reaction time (top 30% vs. bottom 30%). n = 21 sessions (2100 trials), 7 mice. (I) Correlation matrix summarizing the relationship between motif-specific kinematics (Trajectory Travelled Length, Mean/Peak Velocity) and motif specific SNr Z-scores. Circle size denotes statistical significance (p-value). Color intensity indicates the correlation coefficient. Spearman’s rank correlation coefficient was used (Supplementary Table 1). n = 21 sessions (2100 trials), 7 mice. Refer to Table 1 for summary statistics. (J) Representative scatter plots showing significant positive correlations between SNr activity and specific kinematic parameters: Open motif trajectory length (top) and Grasp motif mean velocity (bottom). Colored outlines in (I) indicate the specific correlations displayed here. n = 21 sessions (2100 trials), 7 mice. Pearson correlation coefficient was used.

    Article Snippet: For miniscope calcium imaging, 100 nL of AAV9-Syn-FLEX-jGCaMP7f (Addgene #104488) was injected unilaterally into the left SNr.

    Techniques: Sequencing, Blocking Assay, Injection, Activity Assay

    (A) Schematic of the miniscope recording strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to monitor calcium dynamics in GABAergic projection neurons at single-cell resolution during behavior. (B) Average population activity aligned to the onset of the Reach motif. The solid line represents the mean, and the shaded area represents SEM. (C) Heatmap of trial-averaged, Reach-aligned calcium activity for all recorded neurons. (D) Proportion of neurons exhibiting significantly increased (orange), decreased (purple), or unchanged (gray) activity during specific action motifs compared to baseline periods (Wilcoxon Signed-Rank Test). (E) Heatmap of the change in activity (ΔZscore; Behavior - Baseline) for all neurons across the six action motifs, sorted in descending order by modulation magnitude during the Retract motif. (F) Representative temporal traces comparing actual (orange) and predicted (light purple) forelimb spatial coordinates (X, Y, Z) and velocity. Predictions were generated using a linear regression model trained on concurrent SNr population activity. (G) Quantification of the linear regression model’s performance ($R^2$ score) in predicting moment-by-moment kinematics across sessions. Violin plots display the full data distribution density, with internal box plots representing the median (center marker) and the interquartile range. (H) Heatmap of normalized regression weights for each neuron across the four predicted kinematic features. Neurons (rows) are sorted in descending order by their assigned weight in the velocity model. n = 315 neurons from 600 trials, 10 sessions, 5 mice.

    Journal: bioRxiv

    Article Title: Basal ganglia output dynamically controls skilled forelimb kinematics in real time

    doi: 10.64898/2026.03.09.710687

    Figure Lengend Snippet: (A) Schematic of the miniscope recording strategy. AAV9-Syn-FLEX-jGCaMP7f was injected into the SNr to monitor calcium dynamics in GABAergic projection neurons at single-cell resolution during behavior. (B) Average population activity aligned to the onset of the Reach motif. The solid line represents the mean, and the shaded area represents SEM. (C) Heatmap of trial-averaged, Reach-aligned calcium activity for all recorded neurons. (D) Proportion of neurons exhibiting significantly increased (orange), decreased (purple), or unchanged (gray) activity during specific action motifs compared to baseline periods (Wilcoxon Signed-Rank Test). (E) Heatmap of the change in activity (ΔZscore; Behavior - Baseline) for all neurons across the six action motifs, sorted in descending order by modulation magnitude during the Retract motif. (F) Representative temporal traces comparing actual (orange) and predicted (light purple) forelimb spatial coordinates (X, Y, Z) and velocity. Predictions were generated using a linear regression model trained on concurrent SNr population activity. (G) Quantification of the linear regression model’s performance ($R^2$ score) in predicting moment-by-moment kinematics across sessions. Violin plots display the full data distribution density, with internal box plots representing the median (center marker) and the interquartile range. (H) Heatmap of normalized regression weights for each neuron across the four predicted kinematic features. Neurons (rows) are sorted in descending order by their assigned weight in the velocity model. n = 315 neurons from 600 trials, 10 sessions, 5 mice.

    Article Snippet: For miniscope calcium imaging, 100 nL of AAV9-Syn-FLEX-jGCaMP7f (Addgene #104488) was injected unilaterally into the left SNr.

    Techniques: Injection, Single Cell, Activity Assay, Generated, Marker